KMID : 0545120170270101855
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Journal of Microbiology and Biotechnology 2017 Volume.27 No. 10 p.1855 ~ p.1866
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Specific Expression of Interferon-¥ã Induced by Synergistic Activation Mediator-Derived Systems Activates Innate Immunity and Inhibits Tumorigenesis
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Liu Shuai
Yu Xiao Wang Qiankun Liu Zhepeng Xiao Qiaoqiao Hou Panpan Hu Ying Hou Wei Yang Zhanqiu Guo Deyin Chen Shuliang
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Abstract
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The synergistic activation mediator (SAM) system can robustly activate endogenous gene expression by a single-guide RNA. This transcriptional modulation has been shown to enhance gene promoter activity and leads to epigenetic changes. Human interferon-¥ã is a common natural glycoprotein involved in antiviral effects and inhibition of cancer cell growth. Large quantities of high-purity interferon-¥ã are important for medical research and clinical therapy. To investigate the possibility of employing the SAM system to enhance endogenous human interferon-¥ã with normal function in innate immunity, we designed 10 single-guide RNAs that target 200 bp upstream of the transcription start sites of the interferon-¥ã genome, which could significantly activate the interferon-¥ã promoter reporter. We confirmed that the system can effectively and highly activate interferon-¥ã expression in several humanized cell lines. Moreover, we found that the interferon-¥ã induced by the SAM system could inhibit tumorigenesis. Taken together, our results reveal that the SAM system can modulate epigenetic traits of non-immune cells through activating interferon-¥ã expression and triggering JAK-STAT signaling pathways. Thus, this strategy could offer a novel approach to inhibit tumorigenesis without using exogenous interferon-¥ã.
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KEYWORD
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Synergistic activation mediator (SAM), CRISPR-dCas9, humanized interferon-¥ã, JAK/STAT signaling pathway, tumorigenesis
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